NCIH82_LUNG


Available Data

  • Vendor Karyotype
  • ecDNA Presence Verification from Literature
  • Amplicon Architect Prediction
  • Circle Hunter Prediction
  • Shatterseek Prediction

ID Value
ATCC HTB-175
DSMZ ACC-556
Cellosaurus NCI-H82
Cosmic 688031.0

Property Value
% Polyploidy 3.0
Ploidy Classification hypotriploid
Modal Chromosome Number 58.0
Modal Range Numeric 58-68
Marker Chromosomes Average Field 3.0
Primary or Metastasis Metastasis
Count of SV's 1

Details

ecDNA Present (Y/N/P): Y

Literature source: https://www.sciencedirect.com/science/article/pii/S0165460805001561#bib10
https://aacrjournals.org/cancerdiscovery/article/13/4/928/725029/Extrachromosomal-DNA-Amplification-Contributes-to

HSR Classification: Y

ATCC Karyotype: This is a near triploid human cell line. The modal chromosome number is 58, occurring at 44% with polyploidy at 3%. Marker chromosomes der(1)t(1;709p13;p11), t(13q;?HSR;15q) and der(190t(19;?)(q13.4;?) were common to most cells., There were two distinct subpopulations readily distinguished by karyotype. Besides uniform changes in the numbers of copies of some normal chromosomes, one population had der(3)t(3;20)(p11;p11?), t(3q19p), i(7q) and a minute chromosome of unknown origin., The other had t(1q17p), del(1)(q21), der(3)t(3;7)(p12;q11) plus two other markers. Each cell had two copies of a normal X chromosome. The Y chromosome was not detected in Q banded preparations.

DSMZ Karyotype: No data

Heterogeneity Indications and Other Notes: a minute chromosome of unknown origin There were two distinct subpopulations readily distinguished by karyotype.

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Prediction

ecDNA Amplicon Predicted: Y

Predicted Amplicon Classes: ['ecDNA']

Predicted Genes on ecDNA

['CASC11', 'CASC19', 'CASC21', 'CASC8', 'CCAT1', 'CCAT2', 'MYC', 'POU5F1B', 'BCL2L2', 'BCL2L2-PABPN1', 'CMTM5', 'EFS', 'HOMEZ', 'IL25', 'MYH6', 'MYH7', 'PABPN1', 'PPP1R3E', 'RNF212B', 'SLC22A17']

Predicted Features

Class Amplicon Complexity Score
ecDNA 1 0.9682448035
Image 1

Class Amplicon Complexity Score
ecDNA 1 0.4822271272
Image 2

Prediction

ecDNA: negative


Prediction

Shatterseek Prediction: High Confidence

Shatterseek High Confidence Chromosomes: 2, 3

Shatterseek Low Confidence Chromosomes: 4