NCIH146_LUNG


Available Data

  • Vendor Karyotype
  • ecDNA Presence Verification from Literature
  • Amplicon Architect Prediction
  • Shatterseek Prediction

ID Value
ATCC HTB-173
DSMZ None
Cellosaurus NCI-H146
Cosmic 910899.0

Property Value
% Polyploidy None
Ploidy Classification mix(hypotriploid, triploid)
Modal Chromosome Number 68.0
Modal Range Numeric 66-71
Marker Chromosomes Average Field 15.0
Primary or Metastasis Metastasis
Count of SV's 6

Details

ecDNA Present (Y/N/P): N

Literature source: https://aacrjournals.org/cancerdiscovery/article/13/4/928/725029/Extrachromosomal-DNA-Amplification-Contributes-to

HSR Classification: Y

ATCC Karyotype: This is a near triploid human cell line. The modal chromosome number is 68, but cells with 66, 70 and 71 chromosomes also occurred frequently. At least 15 markers were common to all cells including single copies of der(1)t(1;13)(p32;q12) and del(3)(p21/22), and paired der(7)t(7;?)(p22;?), t(13q16q) and t(16p21q). Ten others were seen in single copies or paired. No HSR or DM were found. Structurally normal N5, N13 and N14 were absent. Four or more copies of N18 and N20 were present in each cell, and N5, N7 and N11 were mostly single copies per cell. The X chromosomes were paired, and no Y chromosome was detected in QM stained preparations.

DSMZ Karyotype: No data

Heterogeneity Indications and Other Notes: No HSR or DM were found

Download Breakpoint Edges CSV

Prediction

ecDNA Amplicon Predicted: Y

Predicted Amplicon Classes: ['Linear amplification', 'ecDNA']

Predicted Genes on ecDNA

['AFDN', 'FRMD1', 'HGC6.3', 'KIF25', 'KIF25-AS1']

Predicted Features

Class Amplicon Complexity Score
Linear amplification 1 0.6073076124
Image 1

Class Amplicon Complexity Score
ecDNA 2 0.6869072775
Image 2

Class Amplicon Complexity Score
Linear amplification 3 1.010240424
Image 3

Prediction

ecDNA: None


Prediction

Shatterseek Prediction: Low Confidence

Shatterseek High Confidence Chromosomes: None

Shatterseek Low Confidence Chromosomes: 6, 11