HT29_LARGE_INTESTINE

Available Data

• Vendor Karyotype
• ecDNA Presence Verification from Literature
• Amplicon Architect Prediction
• Circle Hunter Prediction
• Shatterseek Prediction

IDs from Other Resources

Karyotype Details

Details

ecDNA Present (Y/N/P): N

Literature source: https://www.nature.com/articles/1208834
https://www.nature.com/articles/nature21356

HSR Classification: N

ATCC Karyotype: modal number = 71; range = 68 to 72.The stemline chromosome number is hypertriploid with the 2S component occurring at 2.4%. Seventeen marker chromosomes are found in most metaphases, generally in single copy per chromosome. The marker designations are: M1p-(=t(3p-;?) with a deleted short arm), t(7q;?), t(10q;?), i(13q), 19q+a; M6, ?t(8q;9q-), ?Xp, M9, 6q+, t(13;?)a, t(13;?)b, 19q+b, M14, M15, 15p+, and Xq-. Chromosome 13 is nullisomic and chromosomes 8 and 14 are generally monosomic. No Y chromosome was detected by QM band analysis.

DSMZ Karyotype: human hypertriploid karyotype with 17.5% polyploidy - 71(63-74)<3n>XX, -X, +1, +2, +7, -8, -9, +11, -13, -13, -14, +15, -18, +19, -21, -22, +4-6mar, del(3)(p24), del(4)(q22)x1-2, add(6)(q25), add(7)(p15), i(13q), add(19)(q13) - sideline with i(17q), t(17;19)(p10;q10) - resembles published karyotype and to that of CX-1 which is a subclone - carries very large isochromosomal ABR marker

Heterogeneity Indications and Other Notes: developed MTX resistance, showing amplification of the DHFR gene at 5q12–14 (>20-fold amplification and presence of extrachromosomal double minutes)