DAOY_CENTRAL_NERVOUS_SYSTEM

Available Data

• Vendor Karyotype
• ecDNA Presence Verification from Literature
• Amplicon Architect Prediction
• Shatterseek Prediction

IDs from Other Resources

Karyotype Details

Details

ecDNA Present (Y/N/P): Y

Literature source: https://pubmed.ncbi.nlm.nih.gov/2334901/

HSR Classification: N

ATCC Karyotype: This is a hypertetraploid human cell line with a modal number between 93 and 99. The frequency of cells with higher ploidies is 2.0%. However, since the stemline chromosome number is high, the estimate for the polyploidy is tentative. Thirteen or more marker chromosomes were common to all cells. Of these, many had two to four copies per cell. Among the markers were: t(1q5q), t(13q;?), 15p+, 7q+, der(9)t(3;9)(p21;q34) and eight others. In most cells, the 15p+ has three copies and der(9) has four copies.Some cells have del(1)(p11). Normal N12, N14, N15 and N19 tend to have four or more copies per cell. There are two normal X chromosomes in most cells, but there is no detectable normal Y.

DSMZ Karyotype: No data

Heterogeneity Indications and Other Notes: DAOY was found to have about 5-fold amplifica tion of erbBl. The medulloblastoma cell line MTS had about 150-fold amplification of the N-myc oncogene (Fig.15). As previously reported (6), cell line D341 Med had about 20-fold amplification of c-myc (Fig. 1C). All 3 of the cell lines with amplification of an oncogene had been shown to have dms on cytogenetic analysis prior to the molecular studies (S. H. B., E. C. D.).